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mouse anti rat light chain specific igg hrp  (Jackson Immuno)


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    Structured Review

    Jackson Immuno mouse anti rat light chain specific igg hrp
    Mouse Anti Rat Light Chain Specific Igg Hrp, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+rat+light+chain+specific+igg+hrp/Peroxidase+AffiniPure+Goat+Anti-Rat+IgG%2C+light+chain+specific/10__31083_slash_fbl27119-81-43-50
    Average 94 stars, based on 71 article reviews
    mouse anti rat light chain specific igg hrp - by Bioz Stars, 2026-10
    94/100 stars

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    Incubation:

    Article Title: Inhibition of Cardiac RIP3 Mitigates Early Reperfusion Injury and Calcium-Induced Mitochondrial Swelling without Altering Necroptotic Signalling
    Article Snippet: Briefly, post-electrophoresis, proteins were transferred onto PVDF membranes (Immobilon-P, Merck Millipore) and incubated with primary antibodies against RIP1 (#3493, Cell Signaling Technology, Danvers, MA, USA), RIP3 (#15828, Cell Signaling Technology, Danvers, MA, USA), pThr231/Ser232-RIP3 (ab222320, Abcam, Cambridge, UK), MLKL (ab243142, Abcam, Cambridge, UK), CaMKIIδ (ab181052, Abcam, Cambridge, UK), pThr286-CaMKIIδ (ab171095, Abcam, Cambridge, UK), PGAM5 (ab244218, Abcam, Cambridge, UK), Drp1 (#5391, Cell Signaling Technology, Danvers, MA, USA), pSer616-Drp1 (#4494, Cell Signaling Technology, Danvers, MA, USA), JNK (#9252, Cell Signaling Technology, Danvers, MA, USA), pThr183/Tyr185-JNK (#9255, Cell Signaling Technology, Danvers, MA, USA), BNIP3 (#3769, Cell Signaling Technology, Danvers, MA, USA), NOX2 (ab129068, Abcam, Cambridge, UK), XO (ab109235, Abcam, Cambridge, UK), MnSOD (#13141, Cell Signaling Technology, Danvers, MA, USA), NLRP3 (#15101, Cell Signaling Technology, Danvers, MA, USA), caspase-1 (ab179515, Abcam, Cambridge, UK), IL-1β (ab9722, Abcam, Cambridge, UK) and TNF (ab66579, Abcam, Cambridge, UK). .. Subsequently, membranes were incubated with the following HRP-conjugated secondary antibodies: donkey anti-rabbit IgG (711-035-152, Jackson ImmunoResearch, West Grove, PA, USA), donkey anti-mouse IgG (115-035-174, Jackson ImmunoResearch, West Grove, PA, USA), donkey anti-rat IgG (112-035-175, Jackson ImmunoResearch, West Grove, PA, USA) and mouse anti-rat light chain specific IgG-HRP (112-035-175, Jackson ImmunoResearch, West Grove, PA, USA). .. Signals were detected using enhanced chemiluminescence (Crescendo Luminata, Merck Millipore, Burlington, MA, USA) and captured by a chemiluminescence imaging system (myECL imager, Thermo Scientific, Waltham, MA, USA).

    Article Title: Proteomic Screening of Early Reperfusion in Acute Ischemic Heart and Insights into Mitochondrial-Associated Cell Damage: Role of RIP3
    Article Snippet: Briefly, after electrophoresis, proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Immobilon-P, Merck Millipore) and incubated with various primary antibodies, including apoptosis-inducing factor (AIF) (#4642, Cell Signaling Technology, Danvers, MA, USA), AIM2 (ab119791, Abcam, Cambridge, UK), caspase-1 (ab179515, Abcam, Cambridge, UK), Caspase11 (#14340, Cell Signaling Technology, Danvers, MA, USA), Caspase-3 (#9662, Cell Signaling Technology, Danvers, MA, USA), Caspase-8 (#4790, Cell Signaling Technology, Danvers, MA, USA), CypD (# 78247, Cell Signaling Technology, Danvers, MA, USA), GSDMC (ab225635, Abcam, Cambridge, UK), GSDMD (#46451, Cell Signaling Technology, Danvers, MA, USA), GSDME (#88874, Cell Signaling Technology, Danvers, MA, USA), IL-1β (ab9722, Abcam, Cambridge, UK), IL-33 (ab187060, Abcam, Cambridge, UK), inducible nitric oxide synthase (iNOS) (610432, BD Biosciences, Milpitas, CA, USA), NLRC4 (ab201792, Abcam, Cambridge, UK), NLRP3 (ab263899, Abcam, Cambridge, UK), PARP1 p25 (ab32064, Abcam, Cambridge, UK) and tumour necrosis factor (TNF) (ab66579, Abcam, Cambridge, UK). .. Following this, the membranes were incubated with Horseradish Peroxidase (HRP)-conjugated secondary antibodies: donkey anti-rabbit IgG (711-035-152, Jackson ImmunoResearch, West Grove, PA, USA), donkey anti-mouse IgG (115-035-174, Jackson ImmunoResearch, West Grove, PA, USA), donkey anti-rat IgG (112-035-175, Jackson ImmunoResearch, West Grove, PA, USA) and mouse anti-rat light chain specific IgG-HRP (112-035-175, Jackson ImmunoResearch, West Grove, PA, USA). .. Protein nitration was evaluated directly (35 μg protein/lane) without additional processing steps using a specific antiNO2Tyr antibody (#9691, Cell Signaling Technology, Danvers, MA, USA) on a PVDF membrane.



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